Biodecolorization of methylene blue by mixed cultures of brown-rot fungus Gloeophyllum trabeum and bacterium Bacillus subtilis

This study reported methylene blue (MB) dye decolorization by using mixed consortium culture, brown-rot fungus (BRF) Gloeophyllum trabeum which is known as degradative with bacterium Bacillus subtilis addition. B. were added into G. cultures at variation 2, 4, 6, 8, and 10 mL incubated statically (1 = 9.47 × 108 CFU). All of had ability to decolorize MB (100 mg/L) in potato dextrose broth (PDB) medium during incubation process 30 °C for 7 days. The addition culture showed the highest decolorized, approximately 82%, while only was 11%. metabolites biodecolorization identified characterized LC-TOF/MS, N-(7-(dimethylamino)-1-hydroxy-3H-phenothiazin-3-ylidene)-N-methylmethanaminium; 2-amino-5-(dimethylamino)-3-((3-(dimethylamino) phenyl) sulfinyl) phenol; N-7-(7-(dimethylamino)-1,4,6,9-tetrahydroxy-3H-phenothiazin-3-ylidene)-N-methylmethanaminium. Those results could indicate that BRF efficient wastewater treatment.